Reaction Mechanism of Bacterial Glu-tRNAGln-Dependent Amidotransferase
نویسندگان
چکیده
منابع مشابه
Dual-targeted tRNA-dependent amidotransferase ensures both mitochondrial and chloroplastic Gln-tRNAGln synthesis in plants.
Aminoacyl-tRNAs are generally formed by direct attachment of an amino acid to tRNAs by aminoacyl-tRNA synthetases, but Gln-tRNA is an exception to this rule. Gln-tRNA(Gln) is formed by this direct pathway in the eukaryotic cytosol and in protists or fungi mitochondria but is formed by an indirect transamidation pathway in most of bacteria, archaea, and chloroplasts. We show here that the format...
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In many bacteria and archaea, an ancestral pathway is used where asparagine and glutamine are formed from their acidic precursors while covalently linked to tRNA(Asn) and tRNA(Gln), respectively. Stable complexes formed by the enzymes of these indirect tRNA aminoacylation pathways are found in several thermophilic organisms, and are called transamidosomes. We describe here a transamidosome form...
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tRNA-dependent amidotransferases (AdT) are essential enzymes for protein biosynthesis in many bacteria and in all archaea. As AdT is essential for a number of pathogenic bacteria, and it is absent from mammalian cytoplasm, it is considered as a putative target for novel inhibitors that could be lead compounds to develop a new class of antibiotics. Besides GatFAB of Saccharomyces cerevisiae mito...
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Aminoacyl-tRNA synthetases play a central role in protein synthesis by covalently linking the correct amino acid to the correct tRNA (1). Each aminoacyl-tRNA is then carried to the ribosome where it interacts with the cognate trinucleotide codon on the mRNA and transfers the amino acid onto a growing polypeptide chain. Work on tRNAs and aminoacyltRNA synthetases from bacteria, fungi, plants, an...
متن کاملGenomics: Re-evaluation of translation machinery evolution
Experiments based on genome sequence analysis have revealed unexpected complexity in the evolution of the translation apparatus, including concerted evolution of Gln-tRNA synthetase and Glu-tRNAGln amidotransferase, and a novel, class I Lys-tRNA synthetase shared by archaea and spirochaetes.
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2007
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.47.190